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Similarly, PC was suppressed in E0771 and metM-Wnt lung cells with Pcx-targeting shRNA purchased from Genecopoeia in a psi-LVRU6H vector (GGAGTTGGAAGAGAATTACAC [shPC-B], CCCACAACTTCAACAAGCTCT [shPC-C], and GCTTCGCGCCGTAGTCTTA [Scram]) and were selected for using hygromycin (500 g/ml)

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Glutathione is also an important storage and transport form of reduced sulfur owing to the thiol-containing Cys residue incorporated into the tripeptide and its biosynthesis is strongly dependent on the sulfur assimilatory pathway including necessary feedback inhibition mechanisms 63